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Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (PRYPPDSVRL LGRVVFKLMD GAPSESEKLY SFYDLESNIN KLTEDKKEGL) of Human SMYD3 (NP_073580).
Our Abpromise guarantee covers the use of ab85277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
ab85277 has not yet been referenced specifically in any publications.