概述

  • 产品名称Anti-SMURF1抗体
    参阅全部 SMURF1 一抗
  • 描述
    兔多克隆抗体to SMURF1
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH-conjugated synthetic peptide selected from the N terminal region of human SMURF1 within aa 1~100.

  • 阳性对照
    • Mouse kidney tissue lysate, human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab38866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • 翻译后修饰Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

Anti-SMURF1 antibody 图像

  • Anti-SMURF1 antibody (ab38866) at 1/100 dilution + mouse kidney tissue lysate at 35 µg

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
  • ab22983 at a 1/50 dilution, staining human breast carcinoma by immunohistochemistry by formalin-fixed and paraffin-embedded tissue, followed by AEC staining.

Anti-SMURF1 antibody (ab38866)参考文献

This product has been referenced in:
  • Vimalraj S  et al. A positive role of microRNA-15b on regulation of osteoblast differentiation. J Cell Physiol 229:1236-44 (2014). Read more (PubMed: 24435757) »
  • van Dekken H  et al. Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet 189:37-42 (2009). Read more (PubMed: 19167610) »

See all 3 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (heads)
Loading amount 30 µg
Specification heads
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 17 2012

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I have issued 2 discount codes as part of our "100% testing discount offer". To redeem this offer, please submit your Abreview with the Zebrafish data and include these codes in the "Additional Comments" section so t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (aortic smooth muscle cells)
Loading amount 20 µg
Specification aortic smooth muscle cells
Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12% MOPS running buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"