概述

  • 产品名称
    Anti-SMURF1抗体[1D7]
    参阅全部 SMURF1 一抗
  • 描述
    小鼠单克隆抗体[1D7] to SMURF1
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC/IF, Sandwich ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant Human SMURF1 protein (NP_851994.1).

  • 阳性对照
    • Human Prostate tissue; RAW 264.7 cell lysate; HeLa cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

应用

Our Abpromise guarantee covers the use of ab117552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 83 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

靶标

  • 功能
    E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • 翻译后修饰
    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

图片

  • ELISA of the detection limit for recombinant proprietary tagged SMURF1 is approximately 0.1ng/ml as a capture antibody (ab117552).
  • ab117552 at 10µg/ml dilution staining SMURF1 in HeLa cells by Immunofluorescence.
  • ab117552, at 5 µg/ml, staining SMURF1 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • Immunoprecipitation of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody (ab117552) and Protein A Magnetic Beads.
  • Anti-SMURF1 antibody [1D7] (ab117552) + RAW 264.7 cell lysate

    Predicted band size : 83 kDa
  • All lanes : Anti-SMURF1 antibody [1D7] (ab117552)

    Lane 1 : 293T lysate, transfected with SMURF1 protein
    Lane 2 : 293T lysate, mock transfected


    Predicted band size : 83 kDa

文献

This product has been referenced in:
  • He S  et al. The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function. Sci Rep 7:41364 (2017). Read more (PubMed: 28169289) »
  • Xie P  et al. The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis. Nat Commun 5:3733 (2014). Read more (PubMed: 24821572) »

See all 2 Publications for this product

客户评价及客户问答

As shown on the http://www.abcam.com/ab117552, ab117552has been tested in Mouse and in ICC/IF, it is therefore guaranteed to work in these particularspecies andapplication and we cannot offer a testing discount.

At Abcam, providing quality se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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