概述

  • 产品名称Anti-SMUG1抗体
    参阅全部 SMUG1 一抗
  • 描述
    山羊多克隆抗体to SMUG1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    PQAFLLGSIHEPA-C

    , corresponding to N terminal amino acids 2-14 of Human SMUG1.

  • 阳性对照
    • Human brain cortex tissue; MOLT-4 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab115572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FDG antibody
    • HMUDG antibody
    • MGC104370 antibody
    • Single strand selective monofunctional uracil DNA glycosylase 1 antibody
    • Single strand selective monofunctional uracil DNA glycosylase antibody
    • Single-strand selective monofunctional uracil DNA glycosylase antibody
    • SMUG 1 antibody
    • Smug1 antibody
    • SMUG1 protein antibody
    • SMUG1_HUMAN antibody
    • UNG 3 antibody
    • UNG3 antibody
    see all

Anti-SMUG1 antibody 图像

  • Human Brain Cortex tissue (formalin-fixed, paraffin-embedded) stained with ab115572 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-SMUG1 antibody (ab115572) at 0.5 µg/ml (1 hour incubation) + MOLT-4 cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 29 kDa

Anti-SMUG1 antibody (ab115572)参考文献

ab115572 has not yet been referenced specifically in any publications.

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