Anti-SMUG1抗体(ab104663)

概述

  • 产品名称Anti-SMUG1抗体
    参阅全部 SMUG1 一抗
  • 描述
    兔多克隆抗体to SMUG1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 110-159 of Human SMUG1 (IVGPVLTPPQ EHPKRPVLGL ECPQSEVSGA RFWGFFRNLC GQPEVFFHHC).

  • 阳性对照
    • HeLa cell lysates

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab104663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Suggested dilution in 5% skim milk / PBS buffer Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FDG antibody
    • HMUDG antibody
    • MGC104370 antibody
    • Single strand selective monofunctional uracil DNA glycosylase 1 antibody
    • Single strand selective monofunctional uracil DNA glycosylase antibody
    • Single-strand selective monofunctional uracil DNA glycosylase antibody
    • SMUG 1 antibody
    • Smug1 antibody
    • SMUG1 protein antibody
    • SMUG1_HUMAN antibody
    • UNG 3 antibody
    • UNG3 antibody
    see all

Anti-SMUG1 antibody 图像

  • Anti-SMUG1 antibody (ab104663) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa

Anti-SMUG1 antibody (ab104663)参考文献

ab104663 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepDES19 cells)
Loading amount 100000 cells
Specification HepDES19 cells
Treatment pcDNA3.1 or pcDNA-SMUG1 transfected
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Shijian Zhang

Verified customer

提交于 Oct 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"