概述

  • 产品名称
  • 描述
    兔多克隆抗体to Smt3
  • 特异性
    ab14405 recognizes SUMO-GFP fusion proteins and purified Smt3. It is predicted to bind to yeast SUMO (SMT) bound to other proteins. Cross reactivity with other homologs may occur but has not been specifically tested.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant protein (S. cerevisiae).

  • 阳性对照
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.
  • 常规说明
    Centrifuge product if not completely clear after standing at room temperature.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 12 kDa.
Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
ELISA 1/1000 - 1/5000.

靶标

  • 功能
    Not known; suppressor of MIF2 mutations.
  • 序列相似性
    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Information by UniProt
  • 数据库链接
    • 别名
      • DmSUMO 1 antibody
      • Small Ubiquitin-like modifier antibody
      • SMT3 antibody
      • SMT3_YEAST antibody
      • Ubiquitin like protein of the SUMO family antibody
      • Ubiquitin like protein SMT3 antibody
      • Ubiquitin-like protein SMT3 antibody
      see all

    图片

    • All lanes : Anti-Smt3 antibody (ab14405) at 1/1000 dilution

      Lane 1 : 3 : 5 : 7 : 9 : 11 : 13, Lysate prepared from E.coli expressed Yeast SUMO protein and SUMO-GFP fusion protein after incubation with lysed insect cells
      Lane 2 : 4 : 6 : 8 : 10 : 12 : 14, Lysate prepared from SUMO-GFP fusion protein after incubation with lysed insect cells in the presence of 2% SDS

      Lysates/proteins at 2 µg per lane.
    • ab14405 detecting Smt3 in Escherichia coli recombinant protein (BL21(DE3) by Direct ELISA. Wells were coated with 10µg each of diluted control protein (without SUMO fusion - Negative Controls) and target protein (with SUMO fusion protein) in bicarbonate buffer. Wells were blocked with 5% milk for 1 hour at room temperature. The samples were incubated with primary antibody (1/5000 in TBST +5% milk) for 45 minutes. An HRP-conjugated goat polyclonal to rabbit IgG (1/40000) was used as the secondary antibody.

      See Abreview

    实验方案

    文献

    This product has been referenced in:
    • Neiswinger J  et al. Posttranslational Modification Assays on Functional Protein Microarrays. Cold Spring Harb Protoc 2016:pdb.prot087999 (2016). Read more (PubMed: 27698238) »
    • Bermúdez-López M  et al. Sgs1's roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6. Genes Dev 30:1339-56 (2016). Read more (PubMed: 27298337) »

    See all 4 Publications for this product

    客户评价及客户问答

    The immunogen is full-length protein, untagged, and the amino acid sequence is given at the UniProt entry for S. cerevisiae Smt3,

    http://www.uniprot.org/uniprot/Q12306 .

    This antibody has not been tested by immunprecipitation, or ...

    Read More
    Abreviews
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (log phase yeast cells)
    Loading amount
    100 µg
    Specification
    log phase yeast cells
    Gel Running Conditions
    Reduced Denaturing (6-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Username

    Dr. O Kerscher

    Verified customer

    提交于 Nov 10 2011

    Abreviews
    Application
    ELISA
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Specification
    BL21(DE3)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Type
    Direct
    Username

    Abcam user community

    Verified customer

    提交于 May 28 2010

    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.5 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 26 2010

    Thank you for your enquiry. I am pleased that you are happy with the performance of Smt3 antibody (ab14405). Please can I encourage you to leave an Abreview. This can be done by clicking on the "Abreview" tab on the product datasheet. In return we ...

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    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Please rest ...

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    Thank you for your enquiry. Ab5211 is a Fast-Track antibody and has not yet been fully characterized, therefore we cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide. We do have some prelimin...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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