Anti-SMRC2抗体(ab64853)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SMRC2
  • 特异性
    ab64853 detects endogenous levels of total SMRC2 protein.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal region of human SMRC2.

  • 阳性对照
    • Extracts from HUVEC cells and COLO205 cells.

性能

应用

Our Abpromise guarantee covers the use of ab64853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 133 kDa).
ELISA 1/10000.

靶标

  • 功能
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAF 170 antibody
    • BAF170 antibody
    • BRG1 associated factor 170 antibody
    • BRG1-associated factor 170 antibody
    • Chromatin remodeling complex BAF170 subunit antibody
    • CRACC 2 antibody
    • CRACC2 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 170 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARCC 2 antibody
    • Smarcc2 antibody
    • SMRC2_HUMAN antibody
    • SWI/SNF complex 170 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 2 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2 antibody
    • SWI3 like protein antibody
    see all

图片

  • All lanes : Anti-SMRC2 antibody (ab64853) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from COLO205 cells
    Lane 3 : Extracts from COLO205 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 133 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab64853 has not yet been referenced specifically in any publications.

客户评价及客户问答

Gracias por contactarnos.

He chequeado la homología de dichos anticuerpos con BAF155, y el resultado es el siguiente:

ab64853: Al llevar a cabo un alineamiento de secuencias con BAFF15 (http://www.uniprot.org/uniprot/Q92922) ...

Read More

Thank you for contacting us.  Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab64853. The immunogen sequence range for the antigen is com...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册