Synthetic peptide corresponding to a region within C terminal amino acids 330-360 of Human SMPDL3A, conjugated to KLH.
MDA-MB435 cell line lysates; Human breast carcinoma
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 51 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the acid sphingomyelinase family.
Information by UniProt
Acid sphingomyelinase like phosphodiesterase 3a antibody
Acid sphingomyelinase-like phosphodiesterase 3a antibody
ASM like phosphodiesterase 3a antibody
Western blot - SMPDL3A antibody (ab104049)
Anti-SMPDL3A antibody (ab104049) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg
Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMPDL3A antibody (ab104049)
Immunohistochemical analysis of SMPDL3A expression in formalin fixed and paraffin embedded breast carcinoma, using a 1/50 dilution of ab104049 followed by peroxidase conjugation of the secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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