概述

  • 产品名称Anti-Smoothened抗体
    参阅全部 Smoothened 一抗
  • 描述
    兔多克隆抗体to Smoothened
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human Smoothened.

    (Peptide available as ab39344.)

  • 阳性对照
    • This antibody gave a positive signal against Human Smoothened Partial Recombinant Protein (Tagged)
  • 常规说明ab80683 is raised using the same immunogen as ab38686. Batches sold under ab80683 have passed QC testing in WB (Recombinant only). In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.

性能

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应用

Our Abpromise guarantee covers the use of ab80683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 86 kDa).Can be blocked with Human Smoothened peptide (ab39344).
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-Smoothened antibody 图像

  • Anti-Smoothened antibody (ab80683) at 1 µg/ml + Human Smoothened Partial Recombinant Protein (Tagged) at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 86 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab80683 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80683, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Smoothened antibody (ab80683)参考文献

ab80683 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"