概述

  • 产品名称Anti-Smoothened抗体
    参阅全部 Smoothened 一抗
  • 描述
    兔多克隆抗体to Smoothened
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey, Zebrafish
  • 免疫原

    Synthetic peptide within Human Smoothened aa 190-240. The exact sequence is proprietary. The immunogen sequence is 100% homologous in human ( isoforms CRA_a, CRA_b, CRA_c), rhesus monkey and chimpanzee, 94% in horse, cow and dog, 88% in rat, mouse, chicken, opossum, zebrafish and Xenopus.

  • 阳性对照
    • Human heart lysate Human brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab72130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 24136221ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
  • 序列相似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Frizzled family member 11 antibody
    • FZD11 antibody
    • Gx antibody
    • Gx protein antibody
    • Protein Gx antibody
    • SMO antibody
    • SMO_HUMAN antibody
    • SMOH antibody
    • Smoothened frizzled family receptor antibody
    • Smoothened homolog (Drosophila) antibody
    • Smoothened homolog antibody
    • Smoothened homolog precursor (SMO) (Gx protein) antibody
    • Smoothened seven transmembrane spanning receptor antibody
    see all

Anti-Smoothened antibody 图像

  • All lanes : Anti-Smoothened antibody (ab72130) at 0.1 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate with immunising peptide


    Predicted band size : 86 kDa
    Observed band size : 86 kDa
  • Immunohistochemical analysis using formalin/PFA-fixed paraffin-embedded human brain tissue with an isotype control (top image) and ab72130, at 10µg/ml staining Smoothened (bottom image).
  • ICC/IF image of ab72130 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab72130 staining Smoothened in 6 month-old transgenic zebrafish pancreas (Ihha overexpression) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Sections were incubated with primary antibody (1/200) and HRP-conjugated secondary antibody colored using DAB solution. Slides were counterstained with hematoxylin.

Anti-Smoothened antibody (ab72130)参考文献

This product has been referenced in:
  • Ghezali L  et al. Sonic hedgehog activation is implicated in diosgenin-induced megakaryocytic differentiation of human erythroleukemia cells. PLoS One 9:e95016 (2014). WB ; Human . Read more (PubMed: 24740159) »
  • Im S  et al. Hedgehog related protein expression in breast cancer: gli-2 is associated with poor overall survival. Korean J Pathol 47:116-23 (2013). IHC-P ; Human . Read more (PubMed: 23667370) »

See all 9 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (primary osteoblasts)
Total protein in input 600 µg
Immuno-precipitation step Protein A/G
Specification primary osteoblasts
Username

Dr. Tina Yuan

Verified customer

提交于 Dec 15 2015

Application Western blot
Loading amount 80 µg
Gel Running Conditions Non-reduced Denaturing
Sample Rat Tissue lysate - whole (renal)
Specification renal
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hongfeng Ruan

Verified customer

提交于 Dec 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil & placenta)
Specification tonsil & placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Leica Bond Epitope Retrieval 2
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Apr 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Loading amount 25 µg
Specification HEK293T cells
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse cerebellum)
Loading amount 20 µg
Specification mouse cerebellum
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 29 2012

Thank you for your enquiry regarding ab4058 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

It is very clear from the attached images that the...

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I am very sorry to hear that you have been experiencing problems using our products (ab4058, ab38686, ab72130, ab80645) in the application that you wish.

As our Abpromise indicates, in the event that a product is not functioning in the applic...

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Thank you for contacting us. I have tried to answer your questions below and have attached our IHC-P protocol for your reference.

1) When you do antigen retrieval for ab85898, for how long do you boil in citric buffer?We recommend boiling in...

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Thank you for contacting Abcam today.

I am sorry about the issues you have been having with ab72130 in western blotting. As we discussed on the phone, I am sending you ab113438 as a free of charge replacement, and your order/reference number ...

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