概述

  • 产品名称
    Anti-Smoothened抗体
    参阅全部 Smoothened 一抗
  • 描述
    山羊多克隆抗体to Smoothened
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Dog, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-QSDDEPKRIKKS

    , corresponding to internal sequence amino acids 555-566 of Human Smoothened

  • 阳性对照
    • Human Bone Marrow lysate.

性能

应用

Our Abpromise guarantee covers the use of ab58591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 86 kDa). The observed molecular weight corresponds to findings with ab58574, a product of different design reacting to a different part of the same protein.

靶标

图片

  • Lane 1 : Anti-Smoothened antibody (ab58574) at 0.3 µg/ml (incubated for 1 hour)
    Lane 2 : Anti-Smoothened antibody (ab58591) at 0.3 µg/ml (incubated for 1 hour)

    Lane 1 : Human Bone Marrow lysate (35µg protein in RIPA buffer)
    Lane 2 : Human Bone Marrow lysate (35µg protein in RIPA buffer)

    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab58591 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab58591.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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