Anti-Smoothelin抗体(ab74379)

概述

  • 产品名称Anti-Smoothelin抗体
    参阅全部 Smoothelin 一抗
  • 描述
    兔多克隆抗体to Smoothelin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Smoothelin.

    (Peptide available as ab74378.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human lysates: Liver Tissue, Jurkat Whole Cell, SK N SH Whole Cell, Brain Tissue

性能

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应用

Our Abpromise guarantee covers the use of ab74379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with Human Smoothelin peptide (ab74378).

靶标

Anti-Smoothelin antibody 图像

  • All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 95,100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,45 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

Anti-Smoothelin antibody (ab74379)参考文献

ab74379 has not yet been referenced specifically in any publications.

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Expiration date: 02-March-2013

I am very pleased to hear you would like to test ab74379 with Mouse species. This code will give you 370€ off your next o...

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DISCOUNT CODE: xxxxxxx
Expiration date: 19th of August 2012

I am very pleased to hear you would like to accept our offer and test ab74379 inICC with mouse samples. This code will give you: 1 freeprimary antibodybefore the expiration date...

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Thank you for contacting us in regards to using anti-Smoothelin antibody (ab69724 or ab74379) for ICC of mouse samples.

As discussed over the phone, unfortunately, whilst ab69724 has not been used with mouse samples as yet, ab74379 has only b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"