Anti-Smoothelin抗体(ab74379)
Key features and details
- Rabbit polyclonal to Smoothelin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Smoothelin抗体
参阅全部 Smoothelin 一抗 -
描述
兔多克隆抗体to Smoothelin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig, Orangutan -
免疫原
Synthetic peptide corresponding to Human Smoothelin aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab74378) -
阳性对照
- This antibody gave a positive signal in the following Human lysates: Liver Tissue, Jurkat Whole Cell, SK N SH Whole Cell, Brain Tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74379于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa). |
靶标
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功能
Structural protein of the cytoskeleton. -
组织特异性
Smooth muscle; contractile or vascular (for the long form). -
序列相似性
Belongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain. -
细胞定位
Cytoplasm > cytoskeleton. Exhibits a filamentous organization. - Information by UniProt
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数据库链接
- Entrez Gene: 6525 Human
- Entrez Gene: 29856 Mouse
- Entrez Gene: 289734 Rat
- Omim: 602127 Human
- SwissProt: P53814 Human
- SwissProt: Q921U8 Mouse
- Unigene: 149098 Human
- Unigene: 188516 Mouse
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别名
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
see all
图片
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All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100,95 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This antibody was raised against an immunogen that is predicted to cross react with all isoforms of Smoothelin, with exception to Isoform A (Short).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab74379 尚未被引用在任何文献中。