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Our Abpromise guarantee covers the use of ab53219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 227 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 19729479|
|ICC/IF||Use at an assay dependent concentration.|
Immuhistochemical analysis of paraffin-embedded sections of uterus labelling smoth muscle myosin chain 11 with ab53219 at 1/500 dilution.
This image is courtesy of an Abreview submitted by Dr Mario TorradoLanes 3 and 4: Pig Tissue lysate - Heart
ab53219 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).