Anti-smooth muscle Myosin heavy chain 11抗体(ab53219)

概述

  • 产品名称Anti-smooth muscle Myosin heavy chain 11抗体
    参阅全部 smooth muscle Myosin heavy chain 11 一抗
  • 描述
    兔多克隆抗体to smooth muscle Myosin heavy chain 11
  • 特异性Myosin 11 is highly conserved among vertebrates and this antibody specifically recognises the heavy chain of myosin 11 found in vertebrate smooth muscle, a non striated muscle present in the walls of hollow organs, the vasculature, the respiratory and gastrointestinal tracts, uterus, bladder and eye. This antibody does not cross-react with myosin of skeletal or non muscle origin and can be used to specifically stain cultured smooth muscle cells and identify vascular and other smooth muscle types in tissue sections.
  • 经测试应用适用于: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Cow, Human, Pig
    预测可用于: all other Vertebrates
  • 免疫原

    Bovine tracheal smooth muscle myosin.

性能

应用

Our Abpromise guarantee covers the use of ab53219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 227 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 19729479
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Muscle contraction.
  • 组织特异性Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • 疾病相关Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • 序列相似性Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • 结构域The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 细胞定位Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Anti-smooth muscle Myosin heavy chain 11 antibody 图像

  • Immuhistochemical analysis of paraffin-embedded sections of uterus labelling smoth muscle myosin chain 11 with ab53219 at 1/500 dilution.

  • ab53219 staining smooth muscle Myosin heavy chain 11 in Pig aortic smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. Ab6717 (1/400) was used as the secondary antibody.

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  • Predicted band size : 227 kDa
    Observed band size : 228 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Dr Mario Torrado

    Lanes 3 and 4: Pig Tissue lysate - Heart

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  • ab53219 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

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Anti-smooth muscle Myosin heavy chain 11 antibody (ab53219)参考文献

This product has been referenced in:
  • Krawczyk KK  et al. Assessing the contribution of thrombospondin-4 induction and ATF6a activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction. Sci Rep 6:32449 (2016). WB ; Mouse . Read more (PubMed: 27581066) »
  • Simard E  et al. Receptor for Advanced Glycation End-Products Signaling Interferes with the Vascular Smooth Muscle Cell Contractile Phenotype and Function. PLoS One 10:e0128881 (2015). WB ; Rat . Read more (PubMed: 26248341) »

See all 17 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (Carotid Artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization Yes - 0.1% Triton X-100 for 15 mins
Specification Carotid Artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
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提交于 Oct 20 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (aortic smooth muscle cells)
Gel Running Conditions Reduced Denaturing (15)
Loading amount 10 µg
Specification aortic smooth muscle cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Mr. MC Shen

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提交于 May 30 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Xenopus laevis Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Permeabilization Yes - 1% Triton X100
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Mar 10 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Uterus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH=6.0
Permeabilization No
Specification Uterus
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Formaldehyde
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提交于 Oct 23 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Rat Cell lysate - whole cell (embryonic rat aortic smooth muscle cell line (A7r5)
Specification embryonic rat aortic smooth muscle cell line (A7r5
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Dr. Cynthia Gershome

Verified customer

提交于 Mar 13 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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提交于 Nov 19 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step 1%serum,0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (smooth muscle cells)
Specification smooth muscle cells
Permeabilization Yes - see blocking buffer
Fixative Paraformaldehyde
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提交于 Jun 07 2013


Myofibroblasts are halfway between differentiation from fibroblasts into smooth muscle cells. Thus, certain markers expressed in SMC could be expected to be expressed in myofibroblasts as well. The paper by Jones et al shows the antibody detects m...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Bladder)
Specification Bladder
Fixative 10% Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: .01M Citrate Buffer PH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5000µg/mL · Temperature: 22°C
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提交于 Oct 23 2012

DISCOUNT CODE: ***
Expiration date: December 17, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab53219with human samplesand include this code in the &l...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"