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Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11. The immunogen sequence exists in isoforms 3 and 4 but not isoforms 1 and 2 of the UniProt record P35749.
Our Abpromise guarantee covers the use of ab683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. PubMed: 21103378|
|ICC/IF||1/250. See Abreview.|
|IHC-P||1/400. For paraffin embedded sections, Carnoy's fixative may give better results than formalin.|
ab683 at a 1/250 dilution staining human Primary Aortic Smooth Muscle Cells by Immunocytochemistry. The cells were fixed for 20 minutes with cold acetone, blocked and incubated with the antibody overnight at 4C. Bound antibody was detected using an Alexa Fluor ® 488 conjugated Goat anti-Mouse antibody.
This image is courtesy of an Abreview submitted by Amy Pyle on on 7 March 2006.
ab683 at 1/400 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa Fluor ® 594 conjugated goat anti-mouse IgG1 was used as the secondary.