概述

  • 产品名称Anti-SMG5抗体
    参阅全部 SMG5 一抗
  • 描述
    兔多克隆抗体to SMG5
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human SMG5.

    (Peptide available as ab36628.)

  • 阳性对照
    • Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).

    This antibody does not seem suitable to be used for ICC/IF. Please refer to abreviews for further details.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性SMG5 plays a role in nonsense mediated mRNA decay. It promotes dephosphorylation of RENT1, and is necessary for TERT activity. It is part of a complex that contains SMG1, SMG5, SMG7, PP2AC, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated RENT1/UPF1.
    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • EST1 like protein B antibody
      • EST1B antibody
      • LPTS interacting protein antibody
      • LPTS RP1 antibody
      • Nonsense mediated mRNA decay factor antibody
      see all

    Anti-SMG5 antibody 图像

    • Anti-SMG5 antibody (ab33033) at 1/250 dilution + Jurkat whole cell lysate (ab7899) at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 114 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    • IHC image of ab33033 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33033, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-SMG5 antibody (ab33033)参考文献

    ab33033 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (RPE-1)
    Specification RPE-1
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Sep 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"