Anti-Smg1抗体(ab82548)

概述

  • 产品名称Anti-Smg1抗体
    参阅全部 Smg1 一抗
  • 描述
    兔多克隆抗体to Smg1
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residues 2300 and 2350 of Human Smg1 (Q96QV0)

  • 阳性对照
    • Human breast adenocarcinoma tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity purified using an epitope specific to Smg1 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
    • 组织特异性Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
    • 序列相似性Belongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 HEAT repeat.
      Contains 1 PI3K/PI4K domain.
    • 翻译后修饰Autophosphorylated.
    • 细胞定位Nucleus. Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 61E3.4 antibody
      • ATX antibody
      • hSMG-1 antibody
      • hSMG1 antibody
      • KIAA0421 antibody
      • Lambda interacting protein antibody
      • Lambda-interacting protein antibody
      • Lambda/iota protein kinase C interacting protein antibody
      • Lambda/iota protein kinase C-interacting protein antibody
      • LIP antibody
      • Phosphatidylinositol 3 kinase related kinase antibody
      • Phosphatidylinositol 3 kinase related protein kinase antibody
      • PI 3 kinase related kinase SMG 1 antibody
      • Serine/threonine protein kinase SMG1 antibody
      • Serine/threonine-protein kinase SMG1 antibody
      • SMG-1 antibody
      • smg1 antibody
      • SMG1_HUMAN antibody
      see all

    Anti-Smg1 antibody 图像

    • ab82548, at a 1:250 dilution, staining human Smg1 in breast adenocarcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    Anti-Smg1 antibody (ab82548)参考文献

    ab82548 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your call today. As we discussed, please forward any protocol information that you can provide and I will be happy to refund this purchase for you. In particular, we would like to know: 1) How was the tissue fixed? ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"