概述

  • 产品名称Anti-Smg1抗体
    参阅全部 Smg1 一抗
  • 描述
    小鼠单克隆抗体to Smg1
  • 经测试应用适用于: WB, ELISA, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment, corresponding to amino acids 2922-3032 of Human Smg1 with tag.

  • 阳性对照
    • Hela nuclear extract. Recombinant tagged human Smg1 fragment. Human adrenal gland tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab77304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 410 kDa.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Smg1 is approximately 0.1ng/ml when used as a capture antibody.
IHC-P Use a concentration of 1.5 µg/ml.
Sandwich ELISA Use a concentration of 10 µg/ml.

靶标

  • 功能Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
  • 组织特异性Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
  • 序列相似性Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 1 HEAT repeat.
    Contains 1 PI3K/PI4K domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 61E3.4 antibody
    • ATX antibody
    • hSMG-1 antibody
    • hSMG1 antibody
    • KIAA0421 antibody
    • Lambda interacting protein antibody
    • Lambda-interacting protein antibody
    • Lambda/iota protein kinase C interacting protein antibody
    • Lambda/iota protein kinase C-interacting protein antibody
    • LIP antibody
    • Phosphatidylinositol 3 kinase related kinase antibody
    • Phosphatidylinositol 3 kinase related protein kinase antibody
    • PI 3 kinase related kinase SMG 1 antibody
    • Serine/threonine protein kinase SMG1 antibody
    • Serine/threonine-protein kinase SMG1 antibody
    • SMG-1 antibody
    • smg1 antibody
    • SMG1_HUMAN antibody
    see all

Anti-Smg1 antibody 图像

  • Anti-Smg1 antibody (ab77304) at 5 µg/ml + Recombinant tagged human Smg1 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 410 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-Smg1 antibody (ab77304) at 5 µg/ml + Hela nuclear extract at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 410 kDa
    Observed band size : >250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,23 kDa. We are unsure as to the identity of these extra bands.
  • Immunoperoxidase of ab77304, at 1.5µg/ml, staining Smg1 in formalin-fixed paraffin-embedded human adrenal gland tissue.

Anti-Smg1 antibody (ab77304)参考文献

ab77304 has not yet been referenced specifically in any publications.

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