概述

  • 产品名称Anti-SMEK2抗体
    参阅全部 SMEK2 一抗
  • 描述
    兔多克隆抗体to SMEK2
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to a region between residue 715 and the C-terminus (residue 764) of human SMEK2 (NP_065196.1)

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab70622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol.
  • 细胞定位Cytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions.
  • 数据库链接
  • 别名
    • FLFL2 antibody
    • FLJ31474 antibody
    • KIAA1387 antibody
    • PP4R3 beta antibody
    • PP4R3B antibody
    • PPP4R3 Beta antibody
    • PPP4R3B antibody
    • Protein Phosphatase 4, Regulatory Subunit 3 Beta, antibody
    • PSY2 antibody
    • Serine/threonine-protein phosphatase 4 regulatory subunit 3B antibody
    • SMEK 2 antibody
    • SMEK homolog 2 antibody
    • SMEK homolog 2 suppressor of mek1 (Dictyostelium) antibody
    • SMEK homolog 2 suppressor of mek1 antibody
    • smk1 antibody
    see all

Anti-SMEK2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMEK2 with ab70622 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SMEK2 antibody (ab70622) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab70622 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab70622 at 1 µg/ml.

Anti-SMEK2 antibody (ab70622)参考文献

ab70622 has not yet been referenced specifically in any publications.

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