概述
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产品名称Anti-Smek1抗体
参阅全部 Smek1 一抗 -
描述山羊多克隆抗体to Smek1
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Xenopus laevis, Zebrafish, a wide range of other species
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免疫原
Synthetic peptide:
C-EQQRERQDNPKLD
, corresponding to internal sequence amino acids 645-657 of Human Smek1 -
阳性对照
- Human pancreas lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab63968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
应用说明
Peptide ELISA: antibody detection limit dilution : 1/16000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
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序列相似性Belongs to the SMEK family.
Contains 1 WH1 domain. -
细胞定位Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions.
- Information by UniProt
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数据库链接
- Entrez Gene: 55671 Human
- Entrez Gene: 68734 Mouse
- Entrez Gene: 314388 Rat
- Entrez Gene: 432127 Xenopus laevis
- Entrez Gene: 558578 Zebrafish
- Omim: 610351 Human
- SwissProt: Q6IN85 Human
- SwissProt: Q6P2K6 Mouse
see all -
别名
- FLFL1 antibody
- KIAA2010 antibody
- MEK1 SUPPRESSOR 1 antibody
see all
Anti-Smek1 antibody 图像
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Western blot - Smek1 antibody (ab63968)Anti-Smek1 antibody (ab63968) at 0.3 µg/ml + Human Pancreas lysate at 35 µg
Predicted band size : 95 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Anti-Smek1 antibody (ab63968)参考文献
ab63968 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"