Anti-SMCR7L抗体(ab89944)

概述

  • 产品名称
    Anti-SMCR7L抗体
  • 描述
    兔多克隆抗体to SMCR7L
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (AAMLGIATLA VKRMYDRAIS APTSPTRLSH SGKRSWEEPN WMGSPRLLNR) of human SMCR7L (NP_061881).

  • 阳性对照
    • HT1080 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-SMCR7L antibody (ab89944) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : ~51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~42 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab89944 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Specification
Primary Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Sep 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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