概述

  • 产品名称Anti-SMCHD1抗体
    参阅全部 SMCHD1 一抗
  • 描述
    兔多克隆抗体to SMCHD1
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: DNGRGMTSKQ LNNWAVYRLS KFTRQGDFES DHSGYVRPVP VPRSLNSDIS YFGVGGKQAV FFVGQSARMI SKPADSQDVH ELVLSKED, corresponding to N terminal amino acids 184-271 of Human SMCHD1.

  • 阳性对照
    • U 251 MG cell lysates; Human testis tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

Anti-SMCHD1 antibody 图像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122555 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122555, at 1/50 dilution, staining SMCHD1 in Paraffin Embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-SMCHD1 antibody (ab122555) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

Anti-SMCHD1 antibody (ab122555)参考文献

This product has been referenced in:
  • Sunwoo H  et al. The Xist RNA-PRC2 complex at 20-nm resolution reveals a low Xist stoichiometry and suggests a hit-and-run mechanism in mouse cells. Proc Natl Acad Sci U S A 112:E4216-25 (2015). Mouse . Read more (PubMed: 26195790) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (ES cells)
Specification ES cells
Permeabilization Yes - 0.5% TritonX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 20 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast)
Loading amount 50 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 26 2013

I am very pleased to hear you would like to test ab31865, ab122555, ab136973, and ab154105 in mouse. These codes will give you the value listed above off future orders before the expiration date. To redeem this offer, please submit an Abreview for mous...

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Thank you very much for your interest our products.

To our knowledge, ab31865 and ab122555 have not been tested in mouse. Since we carry over 100,000 products, we are unable to store multiple vial sizes for samples. However by participating ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"