概述

  • 产品名称
  • 描述
    兔多克隆抗体to SMC5
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide:

    FFITPKLLQNLPYSEKMTVLFVYNGPHMLEP

    , corresponding to C terminal amino acids 1050-1101 of Human SMC5, using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137).

  • 阳性对照
    • Nuclear extract from HeLa cells Whole cell lysate from HeLa and 293T cells

性能

应用

Our Abpromise guarantee covers the use of ab18038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa).
IP Use a concentration of 5 - 10 µg/ml.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 相关性
      Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics.
    • 细胞定位
      Nucleus. Chromosome. Chromosome; telomere. Note: Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
    • 数据库链接
    • 别名
      • hSMC 5 antibody
      • hSMC5 antibody
      • KIAA0594 antibody
      • MLZ-453 antibody
      • OTTHUMP00000063367 antibody
      • SMC 5 antibody
      • SMC 5 structural maintenance of chromosomes 5 like 1 antibody
      • SMC protein 5 antibody
      • SMC5 L1 antibody
      • SMC5 protein antibody
      • SMC5 structural maintenance of chromosomes 5 like 1 antibody
      • SMC5L1 antibody
      • Structural maintenance of chromosomes 5 antibody
      • Structural maintenance of chromosomes 5 like 1 antibody
      • Structural maintenance of chromosomes protein 5 antibody
      see all

    图片



    • Predicted band size : 129 kDa


      Ab18038 staining Human SMC5 at a concentration of 0.1 µg/ml by Western Blot, and 5 µg/0.5 mg lysate by Immunoprecipitation.

      Samples:

      A) Nuclear extract (10 and 20 µg) from HeLa cells.

      B) Whole cell lysate (500 µg) from HeLa and 293T cells.

      Detection: Chemiluminescence with exposure times of less than 2 minutes.

    文献

    This product has been referenced in:
    • Gallego-Paez LM  et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell 25:302-17 (2014). Human . Read more (PubMed: 24258023) »
    • Gómez R  et al. Dynamic localization of SMC5/6 complex proteins during mammalian meiosis and mitosis suggests functions in distinct chromosome processes. J Cell Sci 126:4239-52 (2013). Read more (PubMed: 23843628) »

    See all 3 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Nov 02 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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