概述

  • 产品名称Anti-SMC5抗体
    参阅全部 SMC5 一抗
  • 描述
    兔多克隆抗体to SMC5
  • 经测试应用适用于: WB, IPmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide:

    FFITPKLLQNLPYSEKMTVLFVYNGPHMLEP

    , corresponding to C terminal amino acids 1050-1101 of Human SMC5, using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137).

  • 阳性对照
    • Nuclear extract from HeLa cells Whole cell lysate from HeLa and 293T cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab18038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa).
IP Use a concentration of 5 - 10 µg/ml.
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 相关性Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics.
    • 细胞定位Nucleus. Chromosome. Chromosome; telomere. Note: Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
    • 数据库链接
    • 别名
      • hSMC 5 antibody
      • hSMC5 antibody
      • KIAA0594 antibody
      • MLZ-453 antibody
      • OTTHUMP00000063367 antibody
      • SMC 5 antibody
      • SMC 5 structural maintenance of chromosomes 5 like 1 antibody
      • SMC protein 5 antibody
      • SMC5 L1 antibody
      • SMC5 protein antibody
      • SMC5 structural maintenance of chromosomes 5 like 1 antibody
      • SMC5L1 antibody
      • Structural maintenance of chromosomes 5 antibody
      • Structural maintenance of chromosomes 5 like 1 antibody
      • Structural maintenance of chromosomes protein 5 antibody
      see all

    Anti-SMC5 antibody 图像



    • Predicted band size : 129 kDa


      Ab18038 staining Human SMC5 at a concentration of 0.1 µg/ml by Western Blot, and 5 µg/0.5 mg lysate by Immunoprecipitation.

      Samples:

      A) Nuclear extract (10 and 20 µg) from HeLa cells.

      B) Whole cell lysate (500 µg) from HeLa and 293T cells.

      Detection: Chemiluminescence with exposure times of less than 2 minutes.

    Anti-SMC5 antibody (ab18038)参考文献

    This product has been referenced in:
    • Gallego-Paez LM  et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell 25:302-17 (2014). Human . Read more (PubMed: 24258023) »
    • Li J  et al. Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. Neuro Oncol 10:45-51 (2008). Read more (PubMed: 18182627) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Nov 02 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"