概述

  • 产品名称Anti-SMC4抗体- ChIP Grade
    参阅全部 SMC4 一抗
  • 描述
    兔多克隆抗体to SMC4 - ChIP Grade
  • 经测试应用适用于: ChIP, ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide representing aportion of human SMC4 lying within exon 23 (C terminal).

  • 阳性对照
    • 293T cells, normal human lymphoblasts

性能

应用

Our Abpromise guarantee covers the use of ab17958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent dilution. PubMed: 20622854
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 150 kDa).
IP Use at an assay dependent concentration.

靶标

  • 功能Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • 组织特异性Widely expressed. Higher expression in testis, colon, thymus.
  • 序列相似性Belongs to the SMC family. SMC4 subfamily.
  • 结构域The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
  • 细胞定位Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP C antibody
    • CAPC antibody
    • Chromosome associated polypeptide C antibody
    • Chromosome-associated polypeptide C antibody
    • hCAP C antibody
    • hCAP-C antibody
    • SMC 4 antibody
    • SMC protein 4 antibody
    • SMC-4 antibody
    • SMC4 antibody
    • SMC4_HUMAN antibody
    • SMC4L1 antibody
    • Structural maintenance of chromosomes 4 antibody
    • Structural maintenance of chromosomes 4 like 1 antibody
    • Structural maintenance of chromosomes protein 4 antibody
    • XCAP C homolog antibody
    • XCAP-C homolog antibody
    see all

Anti-SMC4 antibody - ChIP Grade 图像



  • Predicted band size : 150 kDa


    ab17958 at 0.2µg/ml detecting SMC4 from 90µg of whole cell lysate from 293T cells (left hand lane) or from normal human lymphoblasts (right hand lane) by western blot. The blot was detected by ECL.
  • ICC/IF image of ab17958 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17958, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • SMC4 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal SMC4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17958.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 200kDa: SMC4; non specific - 65kDa: We are unsure as to the identity of this extra band.

Anti-SMC4 antibody - ChIP Grade (ab17958)参考文献

This product has been referenced in:
  • Pan X  et al. YY1 controls Ig? repertoire and B-cell development, and localizes with condensin on the Ig? locus. EMBO J 32:1168-82 (2013). Read more (PubMed: 23531880) »
  • Huang K  et al. Ribosomal RNA gene transcription mediated by the master genome regulator protein CCCTC-binding factor (CTCF) is negatively regulated by the condensin complex. J Biol Chem 288:26067-77 (2013). WB . Read more (PubMed: 23884423) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. I can confirm that the peptide is conserved in your sequence (GI:30172286). The reference used in selecting peptide antigens for human SMC4 was Swiss Prot entry Q9NTJ3 (the link to this entry can be found on the product data...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"