概述

  • 产品名称
  • 描述
    兔多克隆抗体to SMC4
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide, which represented a portion of human structural maintenance of chromosomes 4 encoded within exons 11 and 12.

  • 阳性对照
    • SMC4 transfected 239T cells.

性能

应用

Our Abpromise guarantee covers the use of ab17957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).
ICC/IF 1/500 - 1/2000.

靶标

  • 功能
    Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • 组织特异性
    Widely expressed. Higher expression in testis, colon, thymus.
  • 序列相似性
    Belongs to the SMC family. SMC4 subfamily.
  • 结构域
    The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
  • 细胞定位
    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP C antibody
    • CAPC antibody
    • Chromosome associated polypeptide C antibody
    • Chromosome-associated polypeptide C antibody
    • hCAP C antibody
    • hCAP-C antibody
    • SMC 4 antibody
    • SMC protein 4 antibody
    • SMC-4 antibody
    • SMC4 antibody
    • SMC4_HUMAN antibody
    • SMC4L1 antibody
    • Structural maintenance of chromosomes 4 antibody
    • Structural maintenance of chromosomes 4 like 1 antibody
    • Structural maintenance of chromosomes protein 4 antibody
    • XCAP C homolog antibody
    • XCAP-C homolog antibody
    see all

图片

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SMC4 with ab17957 at 5µg/ml. Cells were fixed with methanol/actetone and blocked with 3% BSA in PBS. A Rhodamine-conjugated anti-rabbit IgG was used as the secondary antibody. Cells were counterstained with DAPI.


  • Predicted band size : 147 kDa

    Samples: Whole cell lysate (90 µg) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: ab17957 used at a dilution of 1/5,000 and goat anti-rabbit HRP used at a dilution of 1:20,000. Detection: Chemiluminescence with 5 second exposure.

    Samples: Whole cell lysate (90 µg) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: ab17957 used at a dilution of 1/5,000 and goat anti-rabbit HRP used at a dilution of 1:20,000. Detection: Chemiluminescence with 5 second exposure.

文献

ab17957 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Nov 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册