Anti-SMC3 (phospho S383)抗体(ab1277)


  • 产品名称Anti-SMC3 (phospho S383)抗体
    参阅全部 SMC3 一抗
  • 描述
    兔多克隆抗体to SMC3 (phospho S383)
  • 经测试应用适用于: IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Rabbits were immunized with a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes-3 (LocusLink ID 9126)around Serine 383, conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.



Our Abpromise guarantee covers the use of ab1277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明IP: Use at a concentration of 2 - 6 µg/ml.
    This antibody does not work in Western blotting.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
    • 疾病相关Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
    • 序列相似性Belongs to the SMC family. SMC3 subfamily.
    • 结构域The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
      Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
    • 细胞定位Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BAM antibody
      • Bamacan antibody
      • Basement membrane associated chondroitin proteoglycan antibody
      • Basement membrane-associated chondroitin proteoglycan antibody
      • BMH antibody
      • CDLS3 antibody
      • chondroitin sulfate proteoglycan 6 (bamacan) antibody
      • Chondroitin sulfate proteoglycan 6 antibody
      • Chromosome associated polypeptide antibody
      • Chromosome-associated polypeptide antibody
      • CSPG 6 antibody
      • CSPG6 antibody
      • hCAP antibody
      • Human chromosome associated polypeptide antibody
      • im:7142991 antibody
      • SMC 3 antibody
      • SMC protein 3 antibody
      • SMC-3 antibody
      • smc3 antibody
      • SMC3_HUMAN antibody
      • SMC3L1 antibody
      • Structural maintenance of chromosome 3 antibody
      • Structural maintenance of chromosomes 3 antibody
      • Structural maintenance of chromosomes protein 3 antibody
      • u:fb22e01 antibody
      • wu:fc30d07 antibody
      see all

    Anti-SMC3 (phospho S383) antibody 图像

    • Immunoprecipitation:

      • ab1277 (Rabbit polyclonal to phospho SMC 3 (Ser 383)) at a 2.0µg concentration immunoprecipitating pSMC 3; and ab9263 (Rabbit polyclonal to human SMC 3) at a 2.0µg concentration immunoprecipitating SMC 3, each from 200µg of HeLa nuclear cell extract +/- IR treatment. 

      Western Blot of Immunoprecipitation products and also neat Hela nuclear cell extract +/- IR treatment: 

      • Lanes were visualised using ab9263 at a 1:1000 dilution by Western Blot (ECL).

    Anti-SMC3 (phospho S383) antibody (ab1277)参考文献

    ab1277 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization Yes - 0.5% Triton X100 in PBS

    Dr. Kirk McManus

    Verified customer

    提交于 Dec 03 2007

    Thank you for contacting us. It seems to me that the phosphorylation referred to on our datasheet for ab1277, anti-phospho SMC3, is of BRCA1: "SMC3 is involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM". ...

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    Thank you for your email. Can you please indicate the Abcam order number or purchase order number that was used when you purchased ab1277? Thank you, and I look forward to hearing from you.