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Synthetic peptide (Human) conjugated to KLH - which represents a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-3 (GenBank PID 4885399), conjugated toKLH.
Our Abpromise guarantee covers the use of ab9263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 141.5 kDa).|
|ChIP||Use at an assay dependent concentration. PubMed: 20966046|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/200 - 1/800.|
SMC3 signals (green) in a human spermatocyte at the zygotene-pachytene transition stage. Wider signals correspond to synapsed areas, whereas narrower signals show non-synapsed regions.
This image was kindly supplied as part of the review submitted by Maria Oliver-Bonet.
ab9263 Immunoprecipitate in human HeLa cytosolic and nuclear extracts. 500µg of cell lysate incubated with primary antibody (1/100) and matrix (Protein A) in 20mM HEPES ph 7.8, 100mM KAc for 4 hours at 4°C. For Western blotting ab9263 was used at a dilution at 1/1000.
Interphase HeLa cells stained with ab9263 (1/500). The antibody gave predominantly nuclear staining in all interphase cells investigated. ab9263 staining is shown in green. The cells were counterstained with DAPI (red).