概述

  • 产品名称Anti-SMC3抗体
    参阅全部 SMC3 一抗
  • 描述
    小鼠多克隆抗体to SMC3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human SMC3 protein (AAH47324).

  • 阳性对照
    • WB: human colon tissue lysate; SMC3 transfected 293T cell lysate ICC/IF: HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab89618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 142 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
  • 疾病相关Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
  • 序列相似性Belongs to the SMC family. SMC3 subfamily.
  • 结构域The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
  • 细胞定位Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAM antibody
    • Bamacan antibody
    • Basement membrane associated chondroitin proteoglycan antibody
    • Basement membrane-associated chondroitin proteoglycan antibody
    • BMH antibody
    • CDLS3 antibody
    • chondroitin sulfate proteoglycan 6 (bamacan) antibody
    • Chondroitin sulfate proteoglycan 6 antibody
    • Chromosome associated polypeptide antibody
    • Chromosome-associated polypeptide antibody
    • CSPG 6 antibody
    • CSPG6 antibody
    • hCAP antibody
    • Human chromosome associated polypeptide antibody
    • im:7142991 antibody
    • SMC 3 antibody
    • SMC protein 3 antibody
    • SMC-3 antibody
    • smc3 antibody
    • SMC3_HUMAN antibody
    • SMC3L1 antibody
    • Structural maintenance of chromosome 3 antibody
    • Structural maintenance of chromosomes 3 antibody
    • Structural maintenance of chromosomes protein 3 antibody
    • u:fb22e01 antibody
    • wu:fc30d07 antibody
    see all

Anti-SMC3 antibody 图像

  • Anti-SMC3 antibody (ab89618) at 1 µg/ml + human colon tissue lysate at 50 µg

    Predicted band size : 142 kDa
    Observed band size : 142 kDa
    Additional bands at : 46 kDa,50 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SMC3 antibody (ab89618) at 1 µg/ml

    Lane 1 : SMC3 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 142 kDa
    Observed band size : ~142 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of SMC3 in HeLa cell labelled with ab89618 at 10 µg/ml

Anti-SMC3 antibody (ab89618)参考文献

ab89618 has not yet been referenced specifically in any publications.

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