概述

  • 产品名称Anti-SMC2抗体
    参阅全部 SMC2 一抗
  • 描述
    兔多克隆抗体to SMC2
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human structural maintenance of chromosomes 2 encoded within exon 27 (LocusLink ID 10592).

  • 阳性对照
    • Whole cell lysate from mock transfected or SMC2 transfected HEK293T cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab10412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa).
IP Use at 2-10 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/200 - 1/400.

靶标

  • 功能Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • 序列相似性Belongs to the SMC family. SMC2 subfamily.
  • 结构域The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.
  • 细胞定位Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP E antibody
    • CAPE antibody
    • Chromosome associated protein E antibody
    • Chromosome-associated protein E antibody
    • FLJ10093 antibody
    • hCAP E antibody
    • hCAP-E antibody
    • hCAPE antibody
    • PRO0324 antibody
    • SMC 2 antibody
    • SMC protein 2 antibody
    • SMC-2 antibody
    • SMC2 antibody
    • SMC2 L1 antibody
    • SMC2_HUMAN antibody
    • SMC2L1 antibody
    • Structural maintenance of chromosomes (SMC) family member chromosome associated protein E antibody
    • Structural maintenance of chromosomes 2 antibody
    • Structural maintenance of chromosomes 2 like 1 antibody
    • Structural maintenance of chromosomes 2 yeast like 1 antibody
    • Structural maintenance of chromosomes protein 2 antibody
    • XCAP E homolog antibody
    • XCAP-E homolog antibody
    • XCAPE homolog antibody
    see all

Anti-SMC2 antibody 图像



  • Predicted band size : 137 kDa


    ab10412 detection of human SMC2 by Western blot and Immunoprecipitation. Samples: Whole cell lysate (90 µg for WB; 0.5 mg for IP) from HEK293T cells that were mock transfected (E), transfected with full length SMC2 (T for WB), or transfected with truncated SMC2 (T for IP). Antibody: ab10412 used at 0.1 µg/ml for WB and at 5 µg for IP. Detection: Chemiluminescence.
  •  ab10412 (1/1000) staining SMC2 in assynchronous HeLa cells in conjunction with a secondary goat anti-rabbit antibody (1/200) conjugated to Cy3 ® (green). Cells were counterstained with DAPI to show the nucleus (red).

    See Abreview

  • IHC image of ab10412 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10412, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colorectal tissue, staining SMC2 with ab10412 at 1/200 dilution. Staining was detected using DAB.

    N, normal tissue; T: tumor tissue (adenocarcinoma)

Anti-SMC2 antibody (ab10412)参考文献

This product has been referenced in:
  • Xu Z  et al. Quantitative Proteomics Reveals That the Inhibition of Na(+)/K(+)-ATPase Activity Affects S-Phase Progression Leading to a Chromosome Segregation Disorder by Attenuating the Aurora A Function in Hepatocellular Carcinoma Cells. J Proteome Res 14:4594-602 (2015). Read more (PubMed: 26491887) »
  • Huang K  et al. Ribosomal RNA gene transcription mediated by the master genome regulator protein CCCTC-binding factor (CTCF) is negatively regulated by the condensin complex. J Biol Chem 288:26067-77 (2013). WB . Read more (PubMed: 23884423) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Nov 02 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"