概述

  • 产品名称Anti-SMC2抗体
    参阅全部 SMC2 一抗
  • 描述
    兔多克隆抗体to SMC2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Dog, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human structural maintenance of chromosomes 2 encoded within exon 22 (LocusLink ID 10592).

  • 阳性对照
    • Whole cell lysate from mock transfected or SMC2 transfected HEK293T cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab10411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 137 kDa).

靶标

  • 功能Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • 序列相似性Belongs to the SMC family. SMC2 subfamily.
  • 结构域The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.
  • 细胞定位Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP E antibody
    • CAPE antibody
    • Chromosome associated protein E antibody
    • Chromosome-associated protein E antibody
    • FLJ10093 antibody
    • hCAP E antibody
    • hCAP-E antibody
    • hCAPE antibody
    • PRO0324 antibody
    • SMC 2 antibody
    • SMC protein 2 antibody
    • SMC-2 antibody
    • SMC2 antibody
    • SMC2 L1 antibody
    • SMC2_HUMAN antibody
    • SMC2L1 antibody
    • Structural maintenance of chromosomes (SMC) family member chromosome associated protein E antibody
    • Structural maintenance of chromosomes 2 antibody
    • Structural maintenance of chromosomes 2 like 1 antibody
    • Structural maintenance of chromosomes 2 yeast like 1 antibody
    • Structural maintenance of chromosomes protein 2 antibody
    • XCAP E homolog antibody
    • XCAP-E homolog antibody
    • XCAPE homolog antibody
    see all

Anti-SMC2 antibody 图像



  • Predicted band size : 137 kDa


    ab10411 detection of human SMC2 by Western blot. Samples: Whole cell lysate (90 µg) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody: ab10411 used at 0.1 µg/ml. Detection: Chemiluminescence with a 10 minute exposure.

Anti-SMC2 antibody (ab10411)参考文献

ab10411 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast)
Loading amount 25 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"