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Synthetic peptide corresponding to SMC2. The epitope maps to a region between residues 50 and 100 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Our Abpromise guarantee covers the use of ab10399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Detection of human SMC2 by Western Blot. Samples: Whole cell lysate (90 microgram) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody: ab10399 used at 0.1 ug/ml. Detection: Chemiluminescence with a 10 minute exposure.