概述

  • 产品名称Anti-SMC2抗体
    参阅全部 SMC2 一抗
  • 描述
    兔多克隆抗体to SMC2
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human structural maintenance of chromosomes 2 encoded within exon 4 (LocusLink ID 10592).

  • 阳性对照
    • Whole cell lysate from mock transfected or SMC2 transfected HEK293T cells.

性能

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应用

Our Abpromise guarantee covers the use of ab10399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).
ICC/IF 1/200.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • 序列相似性Belongs to the SMC family. SMC2 subfamily.
  • 结构域The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.
  • 细胞定位Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • CAP E antibody
    • CAPE antibody
    • Chromosome associated protein E antibody
    • Chromosome-associated protein E antibody
    • FLJ10093 antibody
    • hCAP E antibody
    • hCAP-E antibody
    • hCAPE antibody
    • PRO0324 antibody
    • SMC 2 antibody
    • SMC protein 2 antibody
    • SMC-2 antibody
    • SMC2 antibody
    • SMC2 L1 antibody
    • SMC2_HUMAN antibody
    • SMC2L1 antibody
    • Structural maintenance of chromosomes (SMC) family member chromosome associated protein E antibody
    • Structural maintenance of chromosomes 2 antibody
    • Structural maintenance of chromosomes 2 like 1 antibody
    • Structural maintenance of chromosomes 2 yeast like 1 antibody
    • Structural maintenance of chromosomes protein 2 antibody
    • XCAP E homolog antibody
    • XCAP-E homolog antibody
    • XCAPE homolog antibody
    see all

Anti-SMC2 antibody 图像



  • Predicted band size : 143 kDa


    Detection of human SMC2 by Western Blot. Samples: Whole cell lysate (90 microgram) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody: ab10399 used at 0.1 ug/ml. Detection: Chemiluminescence with a 10 minute exposure
  • ab10399 (1/200) staining SMC2 in HeLa cells (green). Cells were fixed in methanol, permeabilised with 0.5% triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus(red). Please refer to abreview for further experimental details.

    See Abreview

  • IHC image of ab10399 staining in normal human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SMC2 antibody (ab10399)参考文献

This product has been referenced in:
  • Pan X  et al. YY1 controls Ig? repertoire and B-cell development, and localizes with condensin on the Ig? locus. EMBO J 32:1168-82 (2013). Read more (PubMed: 23531880) »
  • Hancock R Structure of metaphase chromosomes: a role for effects of macromolecular crowding. PLoS One 7:e36045 (2012). ICC/IF . Read more (PubMed: 22540018) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Mouse Cell lysate - whole cell (HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas)
Specification HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2013

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Human Cell lysate - whole cell (HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas)
Specification HEK293, HCT116, U2OS and Mouse Embryonic Fibroblas
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2013

Thank you for contacting us.
As discussed please let me know if you are interested in the 100% Abreview testing discount programme.
Please click the link for more details;
http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapa...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Oct 26 2007

Thanks for your email! It's always great to hear from scientist and hear suggestions for new abs. We don't make a great deal of yeast abs (as you can see from our website) and we usually think that you lucky yeast scientists can tag so easily that ...

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Thank you fo ryour enquiry.Ab10399 is not likely to cross react with the orthologs from S. cerevisiae (SwissProt P38989) or S. pombe (P41003). In no case are the sites well conserved with the orthologs. If you have any further questions then please ge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"