Anti-SMC1L2抗体(ab116373)

概述

  • 产品名称Anti-SMC1L2抗体
    参阅全部 SMC1L2 一抗
  • 描述
    兔多克隆抗体to SMC1L2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 736-765 of Human SMC1L2 (NP_683515.3).

  • 阳性对照
    • Human normal Uterus cell line lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab116373 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 144 kDa.

靶标

  • 功能Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.
  • 序列相似性Belongs to the SMC family. SMC1 subfamily.
  • 结构域The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 or REC8 protein, forming a ring structure.
  • 细胞定位Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. In prophase I stage of meiosis, localizes along the AE of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. Remains chromatin associated at the centromeres up to metaphase II. At anaphase II, dissociates from centromeres, allowing chromosomes segregation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bK268H5 antibody
    • SMC protein 1B antibody
    • SMC-1-beta antibody
    • SMC-1B antibody
    • SMC1 beta antibody
    • SMC1B antibody
    • SMC1B_HUMAN antibody
    • SMC1beta antibody
    • SMC1beta protein antibody
    • Smc1l2 antibody
    • Structural maintenance of chromosomes 1-like 2 antibody
    • Structural maintenance of chromosomes 1B antibody
    • Structural maintenance of chromosomes protein 1B antibody
    see all

Anti-SMC1L2 antibody 图像

  • Anti-SMC1L2 antibody (ab116373) at 1/100 dilution + Human normal uterus cell line lysate at 35 µg

    Predicted band size : 144 kDa

Anti-SMC1L2 antibody (ab116373)参考文献

ab116373 has not yet been referenced specifically in any publications.

Product Wall

I am very sorry to hear that. I have organised for the HeLa nuclear lysate we discussed previously to be sent to you. This is on the order number xxxxxx (purchase order number FOCR xxxxx1). This is being sent to the same address as the original order o...

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That is no problem at all. I'm happy to hear from you.

Can I just confirm that you would still like to recieve the positive control lysate to use with ab116373?

Has there been any improvement in the results observed with this antibo...

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Sorry for the delay in getting back to you.

The lab confirmed that the other anti-SMC1L2 antibody (ab96206) was characterised using the endogenously expressed protein of HeLa, A43, H1299, HeLa, HepG2, Molt-4 and Raji cell lines. These were cu...

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I'm very sorry for the delay in getting back to you, I have unfortunately been taken away from my emails by meetings. I am very sorry for this.

Thank you for letting me know how you have been getting on with the optimisation experiments. It i...

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Thank you for that information.


It seems that the secondary antibody shouldn't be a problem at this dilution if that is what the manufacturer recommends.


Should the suggestions I made not improve the results, please do l...

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Thank you for that extra information.

I now understand much more fully the experiment you have been carrying out. I would like to offer some suggestions to help you improve the results observed so far.

1. I would suggest preparing...

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