Anti-SMC1 (phospho S966)抗体(ab73994)

概述

  • 产品名称Anti-SMC1 (phospho S966)抗体
    参阅全部 SMC1 一抗
  • 描述
    兔多克隆抗体to SMC1 (phospho S966)
  • 特异性ab73994 detects SMC1 only when phosphorylated at serine 966.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phospho-peptide derived from human SMC1 around the phosphorylation site of serine 966 (S-G-SP-Q-R).

  • 阳性对照
    • human lung carcinoma tissue, extracts from HUVEC cells, treated with etoposide (24uM, 24hours)

性能

应用

Our Abpromise guarantee covers the use of ab73994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

靶标

  • Information by UniProt
  • 数据库链接
  • 别名
    • CDLS2 antibody
    • DKFZp686L19178 antibody
    • DXhXs423e antibody
    • DXS423E antibody
    • KIAA0178 antibody
    • MGC138332 antibody
    • OTTHUMP00000061876 antibody
    • RP6 29D12.1 antibody
    • SB1.8 antibody
    • Segregation of mitotic chromosomes 1 antibody
    • Segregation of mitotic chromosomes like 1 antibody
    • SMC 1 antibody
    • SMC protein 1B antibody
    • SMC-1-beta antibody
    • SMC-1B antibody
    • SMC1 antibody
    • SMC1A antibody
    • SMC1alpha antibody
    • SMC1alpha protein antibody
    • SMC1B antibody
    • SMC1B_HUMAN antibody
    • SMC1BETA antibody
    • SMC1beta protein antibody
    • SMC1L1 antibody
    • SMC1L2 antibody
    • SMCB antibody
    • Structural maintenance of chromosome 1 like 1 protein antibody
    • Structural maintenance of chromosome 1 like 2 protein antibody
    • Structural maintenance of chromosomes 1A antibody
    • Structural maintenance of chromosomes 1B antibody
    • Structural maintenance of chromosomes protein 1B antibody
    see all

Anti-SMC1 (phospho S966) antibody 图像

  • All lanes : Anti-SMC1 (phospho S966) antibody (ab73994) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells treated with etoposide (24uM, 24hours),
    Lane 2 : extracts from HUVEC cells, treated with etoposide (24uM, 24hours), with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 143 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <72 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using 1/50 ab73994. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

Anti-SMC1 (phospho S966) antibody (ab73994)参考文献

ab73994 has not yet been referenced specifically in any publications.

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