概述
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产品名称Anti-SMC1 (phospho S957)抗体[EPR2857Y]
参阅全部 SMC1 一抗 -
描述兔单克隆抗体[EPR2857Y] to SMC1 (phospho S957)
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特异性Detects SMC1 only when phosphorylated on serine 957.
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经测试应用适用于: WB, IHC-P, ICCmore details
不适用于: Flow Cyt or IP -
种属反应性与反应: Human
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免疫原
A phospho-peptide corresponding to residues surrounding serine 957 of human SMC1
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阳性对照
- Hela cell lysate; human breast carcinoma or tonsil tissue.
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常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR2857Y
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同种型IgG
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研究领域
相关产品
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab75768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 143 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 27127 Human
- Entrez Gene: 8243 Human
- Omim: 300040 Human
- Omim: 608685 Human
- SwissProt: Q14683 Human
- SwissProt: Q8NDV3 Human
- Unigene: 334176 Human
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别名
- CDLS2 antibody
- DKFZp686L19178 antibody
- DXhXs423e antibody
see all
Anti-SMC1 (phospho S957) antibody [EPR2857Y] 图像
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![Western blot - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)](http://www.abcam.cn/ps/datasheet/Images/75/ab75768/ab75768_1.jpg)
Western blot - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)All lanes : Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution
Lane 1 : Hela cells lysate, untreated
Lane 2 : Hela cells lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size : 143 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)](http://www.abcam.cn/ps/datasheet/Images/75/ab75768/ab75768_6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)ab75768 at 1/100 dilution staining SMC1 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)](http://www.abcam.cn/ps/datasheet/Images/75/ab75768/ab75768_3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)ab75768 at 1/100 dilution staining SMC1 in human tonsil tissue, using a HRP/AP polymerized secondary antibody.
实验方案
Anti-SMC1 (phospho S957) antibody [EPR2857Y] (ab75768)参考文献
ab75768 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"