Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for SMC1 pSer957 were affinity purified using the phosphopeptide immobilized on solid support.
Antibody concentration was determined by extinction coefficient: absorbance at 280nm of 1.4 equals 1.0 mg of IgG.
Segregation of mitotic chromosomes like 1 antibody
SMC 1 antibody
SMC protein 1B antibody
SMC1alpha protein antibody
SMC1beta protein antibody
Structural maintenance of chromosome 1 like 1 protein antibody
Structural maintenance of chromosome 1 like 2 protein antibody
Structural maintenance of chromosomes 1A antibody
Structural maintenance of chromosomes 1B antibody
Structural maintenance of chromosomes protein 1B antibody
Western blot - Anti-SMC1 (phospho S957) antibody (ab1275)
ab1275 at a 1:1000 dilution staining phospho SMC 1 (Ser 957) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract in Western Blot (ECL). ab1275 at a 1:1000 dilution staining phospho SMC 1 (Ser 957) in 100µg of treated (10 Gy IR, 1h) HeLa nuclear extract in Western Blot (ECL).
Mehta K et al. Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators. PLoS Pathog11:e1004763 (2015).
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Horejsí Z et al. Distinct functional domains of Nbs1 modulate the timing and magnitude of ATM activation after low doses of ionizing radiation. Oncogene23:3122-7 (2004).
Read more (PubMed: 15048089) »