Anti-SMC1抗体- ChIP Grade (ab9262)

概述

  • 产品名称Anti-SMC1抗体- ChIP Grade
    参阅全部 SMC1 一抗
  • 描述
    兔多克隆抗体to SMC1 - ChIP Grade
  • 经测试应用适用于: IHC-P, ICC, IP, WB, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH - which represents a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-1 (GenBank PID 5453642).

性能

应用

Our Abpromise guarantee covers the use of ab9262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/200 - 1/800.
IP Use at 1-4 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
ChIP Use at an assay dependent concentration. PubMed: 20966046

靶标

Anti-SMC1 antibody - ChIP Grade 图像

  • All lanes : Anti-SMC1 antibody - ChIP Grade (ab9262) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 143 kDa


    Exposure time : 3 seconds

    Detection: Chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SMC1 with ab9262 at 1/1000 (1µg/ml). Detection: DAB.
  • Interphase HeLa cells stained with ab9262 (1/500). The antibody gave nuclear staining in all interphase nuclei investigated. However,  the antibody failed to recognize any chromatin-associated epitopes in prophase or metaphase cells suggesting that the epitopes may be masked during mitosis. ab9262 staining is shown in green. The cells were counterstained with DAPI (red). 100x magnification.

  • Immunoprecipitation analysis of HeLa whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

    Lanes 1 & 2: ab9262 was used for IP at 6 µg per reaction.
    Lane 3: IP using rabbit anti-SMC1A antibody (ab140493).
    Lane 4: Control IgG.

    For western blotting immunoprecipitated SMC1, ab9262 was used at 1 µg/ml.
    Detection: Chemiluminescence.

  • Ab9262 staining human normal colon tissue. Staining is localised to nuclear compartment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-SMC1 antibody - ChIP Grade (ab9262)参考文献

This product has been referenced in:
  • Qiu Z  et al. Functional interactions between NURF and Ctcf regulate gene expression. Mol Cell Biol 35:224-37 (2015). Read more (PubMed: 25348714) »
  • Mehta K  et al. Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators. PLoS Pathog 11:e1004763 (2015). IP ; Human . Read more (PubMed: 25875106) »

See all 17 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Non-reduced Denaturing (7.5)
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 May 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 50 µg
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC5VA Lung Epithelial Fibroblasts)
Loading amount 15 µg
Specification MRC5VA Lung Epithelial Fibroblasts
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Apr 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 40 µg
Specification HeLa cells
Treatment X radiation (10g)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 May 02 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Dec 07 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - nuclear (Egg extract)
Specification Egg extract
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Dr. Alan Score

Verified customer

提交于 Sep 21 2005

Thank you for your enquiry and I apologize for the shortage that you received in your vial of ab9262. I have arranged for a free of charge replacement vial to be sent to you. It is order reference # 102521 and you will receive it this week. If you h...

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We have not received similar reports from other customers, so I think it is likely that the seal on the vial you received is faulty. We will send you a replacement vial tomorrow.

Thank you for your interest in this antibody. It has only been tested for reactivity to the human ortholog. The 16 aa peptide used to generate and affinity purify this antibody is 100% identical to sequences found in human, mouse, rat, and cow SMC...

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No research has been published yet using this antibody. We welcome feedback from researchers using our antibodies and can provide an Amazon voucher for antibody reviews.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"