Anti-SMC1抗体[C2M] (ab16147)

概述

  • 产品名称
    Anti-SMC1抗体[C2M]
    参阅全部 SMC1 一抗
  • 描述
    小鼠单克隆抗体[C2M] to SMC1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 402-894 of Human SMC1.

  • 阳性对照
    • HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab16147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 143 kDa).

    Several customers have reported the antibody not working well in IHC. We have also had reports that this antibody does not work in ICC/IF on HeLa cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • Information by UniProt
    • 数据库链接
    • 别名
      • CDLS2 antibody
      • DKFZp686L19178 antibody
      • DXhXs423e antibody
      • DXS423E antibody
      • KIAA0178 antibody
      • MGC138332 antibody
      • OTTHUMP00000061876 antibody
      • RP6 29D12.1 antibody
      • SB1.8 antibody
      • Segregation of mitotic chromosomes 1 antibody
      • Segregation of mitotic chromosomes like 1 antibody
      • SMC 1 antibody
      • SMC protein 1B antibody
      • SMC-1-beta antibody
      • SMC-1B antibody
      • SMC1 antibody
      • SMC1A antibody
      • SMC1alpha antibody
      • SMC1alpha protein antibody
      • SMC1B antibody
      • SMC1B_HUMAN antibody
      • SMC1BETA antibody
      • SMC1beta protein antibody
      • SMC1L1 antibody
      • SMC1L2 antibody
      • SMCB antibody
      • Structural maintenance of chromosome 1 like 1 protein antibody
      • Structural maintenance of chromosome 1 like 2 protein antibody
      • Structural maintenance of chromosomes 1A antibody
      • Structural maintenance of chromosomes 1B antibody
      • Structural maintenance of chromosomes protein 1B antibody
      see all

    图片

    • All lanes : Anti-SMC1 antibody [C2M] (ab16147) at 1/1000 dilution

      Lane 1 : Human BT-20 (breast cancer cell line) - whole cell lysate
      Lane 2 : Human BT-474 (breast cancer cell line) - whole cell lysate
      Lane 3 : Human HBL-100 (breast cancer cell line) - whole cell lysate
      Lane 4 : Human HCC-1937 (breast cancer cell line) - whole cell lysate
      Lane 5 : Human HME-1 (breast cancer cell line) - whole cell lysate
      Lane 6 : Human Hs-578T (breast cancer cell line) - whole cell lysate
      Lane 7 : Human MCF-10A (breast cancer cell line) - whole cell lysate
      Lane 8 : Human MDA-MB 157(breast cancer cell line) - whole cell lysate
      Lane 9 : Human MDA-MB 231 (breast cancer cell line) - whole cell lysate
      Lane 10 : Human MDA-MB 436(breast cancer cell line) - whole cell lysate
      Lane 11 : Human MDA-MB 453 (breast cancer cell line) - whole cell lysate
      Lane 12 : Human MDA-MB 549 (breast cancer cell line) - whole cell lysate
      Lane 13 : Human T47D (breast cancer cell line) - whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      HRP conjugated rabbit anti-mouse at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 143 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)


      Exposure time : 6 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    实验方案

    文献

    ab16147 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell lines)
    Loading amount
    50 µg
    Specification
    breast cancer cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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