- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-SMARCD3抗体
参阅全部 SMARCD3 一抗 -
描述兔多克隆抗体to SMARCD3
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宿主Rabbit
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特异性This antibody reacts with SMARCD3
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Zebrafish
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免疫原
A region within synthetic peptide: FERKLDQTIM RKRVDIQEAL KRPMKQKRKL RLYISNTFNP AKPDAEDSDG, corresponding to amino acids 128-177 of Human SMARCD3
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阳性对照
- 293T cell lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab50556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | 1/1.5625e+006. |
靶标
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功能Plays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
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组织特异性Isoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
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序列相似性Belongs to the SMARCD family.
Contains 1 SWIB domain. -
细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 777769 Cow
- Entrez Gene: 6604 Human
- Entrez Gene: 66993 Mouse
- Entrez Gene: 296732 Rat
- Omim: 601737 Human
- SwissProt: Q6STE5 Human
- SwissProt: Q6P9Z1 Mouse
- Unigene: 647067 Human
see all -
别名
- 60 kDa BRG 1/Brm associated factor subunit C antibody
- 60 kDa BRG-1/Brm-associated factor subunit C antibody
- BAF60C antibody
see all
图片
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Western blot - Anti-SMARCD3 antibody (ab50556)Lane 2 : Anti-SMARCD3 antibody (ab50556) at 0.5 µg/ml
Lane 1 : MARKER
Lane 2 : 293T cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 58 kDa (why is the actual band size different from the predicted?)
文献
This product has been referenced in:
- Goljanek-Whysall K et al. myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis. Development 141:3378-87 (2014). WB ; Mouse . Read more (PubMed: 25078649) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"