概述

  • 产品名称Anti-SMARCD3抗体
    参阅全部 SMARCD3 一抗
  • 描述
    兔多克隆抗体to SMARCD3
  • 特异性This antibody reacts with SMARCD3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: FERKLDQTIM RKRVDIQEAL KRPMKQKRKL RLYISNTFNP AKPDAEDSDG, corresponding to amino acids 128-177 of Human SMARCD3

  • 阳性对照
    • 293T cell lysate

应用

Our Abpromise guarantee covers the use of ab50556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.

靶标

  • 功能Plays a role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Stimulates nuclear receptor mediated transcription. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • 组织特异性Isoform 2 and isoform 1 are expressed in brain, heart, kidney, placenta, prostate, salivary gland, spleen, testis, thyroid, trachea and uterus. Isoform 1 is also expressed in skeletal muscle and adipose tissue.
  • 序列相似性Belongs to the SMARCD family.
    Contains 1 SWIB domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60 kDa BRG 1/Brm associated factor subunit C antibody
    • 60 kDa BRG-1/Brm-associated factor subunit C antibody
    • BAF60C antibody
    • BRG1 associated factor 60C antibody
    • BRG1-associated factor 60C antibody
    • Chromatin remodeling complex BAF60C subunit antibody
    • CRACD3 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 60C antibody
    • MGC111010 antibody
    • Rsc6p antibody
    • Smarcd3 antibody
    • SMRD3_HUMAN antibody
    • SWI/SNF complex 60 kDa subunit C antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 3 antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3, isoform 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 antibody
    • Swp73 like protein antibody
    see all

Anti-SMARCD3 antibody 图像

  • Lane 1 :
    Lane 2 : Anti-SMARCD3 antibody (ab50556) at 0.5 µg/ml

    Lane 1 : MARKER
    Lane 2 : 293T cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 55 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

Anti-SMARCD3 antibody (ab50556)参考文献

This product has been referenced in:
  • Goljanek-Whysall K  et al. myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis. Development 141:3378-87 (2014). WB ; Mouse . Read more (PubMed: 25078649) »

See 1 Publication for this product

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