概述

  • 产品名称Anti-SMARCD1抗体
    参阅全部 SMARCD1 一抗
  • 描述
    兔多克隆抗体to SMARCD1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 180-229 (RKLRIFISNT FNPAKSDAED GEGTVASWEL RVEGRLLEDS ALSKYDATKQ) of Human SMARCD1 (NP_003067)

  • 阳性对照
    • Fetal liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

  • 功能Involved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 组织特异性Expressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta.
  • 序列相似性Belongs to the SMARCD family.
    Contains 1 SWIB domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60 kDa BRG-1/Brm-associated factor subunit A antibody
    • 60 kDa BRG1/Brm associated factor subunit A antibody
    • BAF60A antibody
    • BRG1 associated factor 60A antibody
    • BRG1-associated factor 60A antibody
    • Chromatin remodeling complex BAF60A subunit antibody
    • CRACD1 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 60A antibody
    • Rsc6p antibody
    • SMARCD1 antibody
    • SMRD1 antibody
    • SMRD1_HUMAN antibody
    • SWI/SNF complex 60 kDa subunit A antibody
    • SWI/SNF complex 60 kDa subunit antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin d1 antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 antibody
    • Swp73 like protein antibody
    see all

Anti-SMARCD1 antibody 图像

  • Anti-SMARCD1 antibody (ab83208) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

Anti-SMARCD1 antibody (ab83208)参考文献

This product has been referenced in:
  • Goljanek-Whysall K  et al. myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis. Development 141:3378-87 (2014). WB ; Mouse . Read more (PubMed: 25078649) »
  • Xu L  et al. MicroRNA-7-regulated TLR9 signaling-enhanced growth and metastatic potential of human lung cancer cells by altering the phosphoinositide-3-kinase, regulatory subunit 3/Akt pathway. Mol Biol Cell 24:42-55 (2013). WB . Read more (PubMed: 23135998) »

See all 2 Publications for this product

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