概述

  • 产品名称Anti-SMARCC1抗体
    参阅全部 SMARCC1 一抗
  • 描述
    兔多克隆抗体to SMARCC1
  • 特异性ab26304 recognises SMARCC1.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    A region within synthetic peptide: SEEKETEENK ELTDTCKERE SDTGKKKVEH EISEGNVATA AAAALASAAT, corresponding to C terminal amino acids 829-878 of Human SMARCC1

  • 阳性对照
    • Jurkat cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab26304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 123 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • 组织特异性Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
  • 序列相似性Belongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • 翻译后修饰Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI115498 antibody
    • BAF 155 antibody
    • BAF155 antibody
    • BRG 1 associated factor 155 antibody
    • BRG1 associated factor 155 antibody
    • BRG1-associated factor 155 antibody
    • Chromatin remodeling complex BAF155 subunit antibody
    • CRACC 1 antibody
    • CRACC1 antibody
    • Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARC C1 antibody
    • SMARCC 1 antibody
    • SMARCC1 antibody
    • SMRC1_HUMAN antibody
    • SRG 3 antibody
    • SRG3 antibody
    • SWI 3 antibody
    • SWI/SNF complex 155 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
    • SWI3 antibody
    see all

Anti-SMARCC1 antibody 图像

  • Anti-SMARCC1 antibody (ab26304) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 123 kDa
    Observed band size : 123 kDa

Anti-SMARCC1 antibody (ab26304)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (human osteosarcoma cells, U2OS cells)
Loading amount 20 µg
Specification human osteosarcoma cells, U2OS cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Sonia Rocha

Verified customer

提交于 Sep 19 2006

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - whole cell (thymocyte)
Specification thymocyte
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: PFA
Detection step Real-time PCR
Positive control CD4 silencer
Negative control gapdh
Username

Dr. tian chi

Verified customer

提交于 Jun 23 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"