Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/16000 - 1/64000.
Use at an assay dependent concentration.
功能ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.
组织特异性Ubiquitously expressed, with high levels in testis.
疾病相关Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.
序列相似性Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily. Contains 2 HARP domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
ab115611, at 5µg/ml, staining SmarcAL1 in Formalin-fixed, Paraffin-embedded Human brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-SmarcAL1 antibody (ab115611)参考文献
has not yet been referenced specifically in any publications.