概述

  • 产品名称
    Anti-SMARCAD1抗体
    参阅全部 SMARCAD1 一抗
  • 描述
    小鼠多克隆抗体to SMARCAD1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length SMARCAD1 protein (Human)

  • 阳性对照
    • SMARCAD1 transfected 293T cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).
ICC/IF 1/250.

靶标

  • 功能
    Probable ATP-dependent DNA helicase.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 CUE domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent helicase 1 antibody
    • ATP-dependent helicase 1 antibody
    • ETL1 antibody
    • hHEL1 antibody
    • Smarcad1 antibody
    • SMRCD_HUMAN antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 antibody
    see all

图片

  • All lanes : Anti-SMARCAD1 antibody (ab67548) at 1/500 dilution

    Lane 1 : SMARCAD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 117 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • 4-cell stage mouse embryos were recovered and the zona pellucida removed. ab67548 at 1:250 dilution was used to detect SMARCAD1, and as secondary antibldy embryos were incubated in Alexa-fluor488 conjugated donkey anti-mouse IgG. Embryos were then imaged by fluorescence confocal microscopy and projected confocal stack images generated - as shown in two examples. Please see accompanying abreview for additional information.

    See Abreview

文献

This product has been referenced in:
  • Xiao S  et al. SMARCAD1 Contributes to the Regulation of Naive Pluripotency by Interacting with Histone Citrullination. Cell Rep 18:3117-3128 (2017). Read more (PubMed: 28355564) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Pre-implantation mouse embryos (4 cell stage))
Specification
Pre-implantation mouse embryos (4 cell stage)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5 TritonX-100
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Dr. Alex Bruce

Verified customer

提交于 Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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