概述

  • 产品名称Anti-SMARCA2 / BRM抗体
    参阅全部 SMARCA2 / BRM 一抗
  • 描述
    兔多克隆抗体to SMARCA2 / BRM
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    KEDVEKAKKRRGRPPAE

    , corresponding to amino acids 1354-1370 of SMARCA2 conjugated to maleimide-activated KLH via an N-terminal added cysteine.

  • 阳性对照
    • WB: Nuclear extracts of SMARCA2 transfected 293T cell line. ICC: Paraformaldehyde/Triton fixed SMARCA2 transfected 293T cell line.
  • 常规说明Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab12165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明IF(ICC): Use at a concentration of 1 µg/ml on paraformaldehyde/Triton fixed SMARCA2-transfected 293T cell line). Indirect IF.
    WB: Use at a concentration of 0.15 µg/ml (determined using nuclear
    extracts of hBrm-transfected 293T cells). Predicted molecular weight: 230 kDa. Staining of SMARCA2 is specifically inhibited by the immunizing peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
    • 疾病相关Nicolaides-Baraitser syndrome
    • 序列相似性Belongs to the SNF2/RAD54 helicase family.
      Contains 1 bromo domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HSA domain.
      Contains 1 QLQ domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP dependent helicase SMARCA2 antibody
      • ATP-dependent helicase SMARCA2 antibody
      • BAF190 antibody
      • BAF190B antibody
      • BRG1-associated factor 190B antibody
      • BRM antibody
      • FLJ36757 antibody
      • Global transcription activator homologous sequence antibody
      • hBRM antibody
      • hSNF2a antibody
      • MGC74511 antibody
      • Possible global transcription activator SNF2L2 antibody
      • Probable global transcription activator SNF2L2 antibody
      • Protein brahma homolog antibody
      • SMARCA2 antibody
      • SMCA2_HUMAN antibody
      • SNF2 alpha antibody
      • SNF2 like 2 antibody
      • SNF2-alpha antibody
      • SNF2/SWI2 like protein 2 antibody
      • SNF2L2 antibody
      • SNF2LA antibody
      • Sth1p antibody
      • Sucrose nonfermenting 2 like protein 2 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 antibody
      see all

    Anti-SMARCA2 / BRM antibody (ab12165)参考文献

    This product has been referenced in:
    • Nan X  et al. Interaction between chromatin proteins MECP2 and ATRX is disrupted by mutations that cause inherited mental retardation. Proc Natl Acad Sci U S A 104:2709-14 (2007). WB ; Mouse . Read more (PubMed: 17296936) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"