The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IF(ICC): Use at a concentration of 1 µg/ml on paraformaldehyde/Triton fixed SMARCA2-transfected 293T cell line). Indirect IF.
WB: Use at a concentration of 0.15 µg/ml (determined using nuclear
extracts of hBrm-transfected 293T cells). Predicted molecular weight: 230 kDa. Staining of SMARCA2 is specifically inhibited by the immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Global transcription activator homologous sequence antibody
Possible global transcription activator SNF2L2 antibody
Probable global transcription activator SNF2L2 antibody
Protein brahma homolog antibody
SNF2 alpha antibody
SNF2 like 2 antibody
SNF2/SWI2 like protein 2 antibody
Sucrose nonfermenting 2 like protein 2 antibody
SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 antibody
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 antibody
Anti-SMARCA2 / BRM antibody (ab12165)参考文献
This product has been referenced in:
Nan X et al. Interaction between chromatin proteins MECP2 and ATRX is disrupted by mutations that cause inherited mental retardation. Proc Natl Acad Sci U S A104:2709-14 (2007).
Read more (PubMed: 17296936) »