The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 64 kDa).
B5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. Some cellular antigens are associated with EEV and these vary with the cell type used to grow the virus. B5R consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, and for EEV formation.
EEV type I membrane glycoprotein antibody
Western blot - Smallpox B5R antibody (ab53309)
All lanes : Anti-Smallpox B5R antibody (ab53309) at 0.1 µg/ml
Lane 1 : recombinant fusion protein containing amino acids 180-194 Lane 2 : fusion partner only