The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 16 kDa).
相关性Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. There are two major forms of infectious orthopoxvirus: the intracellular mature virion (IMV), which is infectious when released from disrupted cells, and the extracellular enveloped virion (EEV), which buds from infected cells. A27L is an IMV-specific gene.
细胞定位Virion membrane. Note=Envelope fraction of virions.
Anti-Smallpox A27L antibody 图像
Western blot - Smallpox A27L antibody (ab53311)
All lanes : Anti-Smallpox A27L antibody (ab53311) at 0.1 µg/ml
Lane 1 : recombinant fusion protein containing
amino acids 18-32 Lane 2 : fusion partner only
Belin LJ et al. An oncolytic vaccinia virus expressing the human sodium iodine symporter prolongs survival and facilitates SPECT/CT imaging in an orthotopic model of malignant pleural mesothelioma. Surgery154:486-95 (2013).
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