Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 54-84 of Human SMAGP
K562 cell line lysates; Human breast carcinoma tissue.
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 11 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in epithelial cell-cell contacts. May play a role in tumor invasiveness and metastasis formation.
Detected in breast, endometrium, colon and biliary tract. Detected in polarized epithelial structures characterized by cell-cell adhesion (at protein level).
Belongs to the SMAGP family.
O-glycosylated. The O-glycan is modified with sialic acid residues.
Cell membrane. Cytoplasmic vesicle membrane. Predominantly on lateral parts of the membrane, at cell-cell epithelial junctions. Detected on cytoplasmic membranes in undifferentiated tumors.
Information by UniProt
Western blot - SMAGP antibody (ab104059)
Anti-SMAGP antibody (ab104059) at 1/100 dilution + K562 cell line lysate at 35 µg
Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMAGP antibody (ab104059)
Immunohistochemistry analysis of SMAGP expression in formalin fixed and paraffin embedded Human breast carcinoma tissue using 1/50 an104059 followed by a peroxidase conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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