Mouse, Rat, Chicken, Pig
, corresponding to internal sequence amino acids 166-179 of Human SMAD9.
Human kidney tissue and Human skin lysate
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 52 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
Pulmonary hypertension, primary, 2
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.
Information by UniProt
MAD homolog 9 antibody
Mothers against decapentaplegic antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD9 antibody (ab115900)
ab115900, at 5 µg/ml, staining SMAD9 in formalin fixed, paraffin embedded Human kidney by Immunohistochemistry. Developed using biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-SMAD9 antibody (ab115900)
Anti-SMAD9 antibody (ab115900) at 0.3 µg/ml + Human skin lysate in RIPA buffer at 35 µg
Developed using the ECL technique. Predicted band size: 52 kDa Primary incubation was 1 hour.
has not yet been referenced specifically in any publications.
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