概述

  • 产品名称Anti-SMAD6抗体
    参阅全部 SMAD6 一抗
  • 描述
    兔多克隆抗体to SMAD6
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SMAD6.

    (Peptide available as ab66351.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS.

性能

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应用

Our Abpromise guarantee covers the use of ab63713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

靶标

  • 功能Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.
  • 组织特异性Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
  • 序列相似性Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • 翻译后修饰Phosphorylated by BMP type 1 receptor kinase and by PRKX.
    Ubiquitinated by WWP1.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSMAD6 antibody
    • MAD homolog 6 antibody
    • MADH6 antibody
    • MADH7 antibody
    • Mothers against decapentaplegic homolog 6 antibody
    • Mothers against DPP homolog 6 antibody
    • SMAD 6 antibody
    • SMAD family member 6 antibody
    • SMAD mothers against DPP homolog 6 antibody
    • Smad6 antibody
    • SMAD6_HUMAN antibody
    see all

Anti-SMAD6 antibody 图像

  • All lanes : Anti-SMAD6 antibody (ab63713) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 130 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Anti-SMAD6 antibody (ab63713)参考文献

This product has been referenced in:
  • Chen RY  et al. Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells. World J Gastroenterol 20:14895-903 (2014). WB ; Human . Read more (PubMed: 25356049) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Isolated Primary NK Cells)
Gel Running Conditions Reduced Denaturing
Loading amount 250000 cells
Treatment Rest, IL15, TGFB, IL12, IL18 for 24 hrs
Specification Isolated Primary NK Cells
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Sian Drake

Verified customer

提交于 May 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"