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RabMAb

Anti-SMAD5 (phospho S463 + S465)抗体[EP728(2)AY] (ab76296)

概述

  • 产品名称
    Anti-SMAD5 (phospho S463 + S465)抗体[EP728(2)AY]
    参阅全部 SMAD5 一抗
  • 描述
    兔单克隆抗体[EP728(2)AY] to SMAD5 (phospho S463 + S465)
  • 特异性
    Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467).
  • 经测试应用
    适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human SMAD5.

  • 阳性对照
    • HeLa cytoplasmic or nuclear lysate treated with BMP-4 HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab76296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 52 kDa.
ICC/IF 1/50 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能
      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
    • 组织特异性
      Ubiquitous.
    • 序列相似性
      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • 翻译后修饰
      Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • 细胞定位
      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp781C1895 antibody
      • DKFZp781O1323 antibody
      • Dwfc antibody
      • hSmad5 antibody
      • JV5 1 antibody
      • JV5-1 antibody
      • MAD homolog 5 antibody
      • MAD, mothers against decapentaplegic homolog 5 antibody
      • MADH 5 antibody
      • MADH5 antibody
      • Mothers against decapentaplegic homolog 5 antibody
      • mothers against decapentaplegic, drosophila, homolog of, 5 antibody
      • Mothers against DPP homolog 5 antibody
      • MusMLP antibody
      • SMA and MAD related protein 5 antibody
      • SMAD 5 antibody
      • SMAD family member 5 antibody
      • SMAD, mothers against DPP homolog 5 antibody
      • Smad5 antibody
      • SMAD5_HUMAN antibody
      see all

    图片

    • All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilution

      Lane 1 : HeLa cell lysate - untreated
      Lane 2 : HeLa cell lysate - treated with BMP-4

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
    • Immunofluorescent staining of HeLa cells using ab76296 at a 1/50-1/100 dilution.

    文献

    This product has been referenced in:
    • Ghiabi P  et al. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med 13:27 (2015). WB ; Human . Read more (PubMed: 25623554) »
    • Nili M  et al. Soluble Repulsive Guidance Molecule c/Hemojuvelin Is a Broad Spectrum Bone Morphogenetic Protein (BMP) Antagonist and Inhibits both BMP2- and BMP6-mediated Signaling and Gene Expression. J Biol Chem 285:24783-92 (2010). WB ; Human . Read more (PubMed: 20530805) »

    See all 2 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human prostate and cervical cancer cell)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    30 µg
    Specification
    Human prostate and cervical cancer cell
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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