Mouse, Rat, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SMAD5.
(Peptide available as ab100821.)
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; MCF7.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Information by UniProt
- DKFZp781C1895 antibody
- DKFZp781O1323 antibody
- Dwfc antibody
Western blot - SMAD5 antibody (ab88559)
All lanes :
Anti-SMAD5 antibody (ab88559) at 1 µg/mlLane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 :
MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size :
52 kDaObserved band size :
52 kDaExposure time :
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"